The Effect of Oral Administration of the Antibacterial Peptide MPX on Intestinal Inflammation of Mice in Experimental Infection with Escherichia Coli Strain O157: H7

Escherichia coli is a gram-negative bacterium, an intestinal pathogen that can cause inflammation. Antimicrobial peptides are class of small molecule peptides, which has good antibacterial activity against variety gram-positive and negative bacteria. In this regard, the authors aimed to study effect antimicrobial peptide MPX, was administered orally, on wall mice infected with barrier function, were E. coli. Synthesis purification MPX (H-INWKGIAAMAKKLL-NH2) performed by Jier Sheng Hua (Shanghai, China). Mass spectrometry liquid chromatography (HPLC) used for purpose. Coli (O157: H7 ATCC43889) isolated from human faeces in haemolytic uremic syndrome reported. Solid LB agar (Solarbio, China) isolate enterohemorrhagic The culture obtained Chinese Institute Veterinary Drug Control (Beijing, results necropsy found using orally could alleviate damage liver, spleen, lungs less affected. According H&E case group considerably relieved duodenum organs day 7 28. qRT-PCR showed reduce inflammation-related factors mRNA expression IL-2 IL-6 TNF-α addition, significantly increase them RNA tight junction proteins Occludin Zo-1 immune histochemistry further MUC2 jejunum. above attack tract mice, relieve inflammation, improve function. This lays theoretical foundation adding food. see reducing factors, thereby alleviating inflammation caused infection mice. Authors add shows protein intestines improves food function clinical practices